Molecular Genetic Epidemiology A Laboratory Perspective
This volume describes high-throughput approaches to a series of robust, established methodologies in molecular genetic studies of population samples. Such developments have been essential not only to linkage and association studies of single-gene and complex traits in humans, animals and plants, but...
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Format: | eBook |
Language: | English |
Published: |
Berlin, Heidelberg
Springer Berlin Heidelberg
2002, 2002
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Edition: | 1st ed. 2002 |
Series: | Principles and Practice
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Subjects: | |
Online Access: | |
Collection: | Springer Book Archives -2004 - Collection details see MPG.ReNa |
Table of Contents:
- 6 The Special Case of HLA Genes: Detection and Resolution of Multiple Polymorphic Sites in a Single Gene
- 6.1 Introduction
- 6.2 The HLA System
- 6.3 Requirement for HLA Genotyping: Historical Perspectives
- 6.4 PCR-based Approaches to HLA Genotyping
- 6.5 A PCR-SSOP-Based Strategy for HLA Class II Typing Using DNA Derived from Archival Tissue Banks
- 6.6 Concluding Remarks
- References
- 7 Microplate Array Diagonal Gel Electrophoresis (MADGE) Methodologies: The First Five Years
- 7.1 Introduction
- 7.2 Microplate Array Diagonal Gel Electrophoresis (MADGE)
- 7.3 CpG-PCR
- 7.4 Temporal Thermal Ramp MADGE Electrophoresis (Melt-MADGE)
- 7.5 Software Applications (Applies to All MADGE Not Just Melt-MADGE)
- 7.6 Future Developments
- References
- 8 The Use of Sequence Analysis for Homozygote and Heterozygote Base Variation Discovery
- 8.1 Introduction
- 8.2 Sequence Analysis: a Polymorphism Discovery Method
- 8.3 Key Advancements in Sequencing Technologies: Chemistries, Hardware, and Software
- 8.4 Heterozygote and Homozygote Polymorphism Base Calling
- 8.5 Strategies for Sequencing-Based PolymorphismDetection
- 8.6 Summary and Outlook: To Sequence or Not To Sequence — This Is Not in Question
- References
- 3.7 Future Developments
- 3.8 Applications
- References
- 4Minisatellite and Microsatellite DNA Fingerprinting
- 4.1 Introduction
- 4.2 Blood Grouping
- 4.3 DNA Fingerprinting — The Discovery
- 4.4 DNA Fingerprinting — The Applications
- 4.5 An Explosion of DNA Fingerprinting Sequences
- 4.6 Shortcomings of Multilocus Probes
- 4.7 Single Locus Probes — DNA Profiling
- 4.8 Europe and the USA Disagree
- 4.9 Lessons from the Castro Case
- 4.10 Impact of PCR
- 4.11 Digital DNA Typing
- 4.12 Short Tandem Repeat Profiling
- 4.13 The UK National DNA Database
- 4.14 STR Profiling Applications
- 4.15 The Future
- References
- 5 Multiplex Polymerase Chain Reaction and Immobilized Probes: Application to Cardiovascular Disease
- 5.1 Introduction
- 5.2 Multiplex Polymerase Chain Reaction (PCR) Methodology
- 5.3 Sequence-Specific Oligonucleotide Probe (SSOP) Methodology
- 5.4 Example: Candidate Markers of CVD Risk
- 5.5 Summary
- References
- 1 Mapping Genes for Common Diseases: Statistical Planning, Power, Efficiency and Informatics
- 1.1 Introduction
- 1.2 Power and Sampling Considerations
- 1.3 Models of Locus Action
- 1.4 Maximum Likelihood Estimation
- 1.5 Allelic Association
- 1.6 The Malecot Model for Association
- 1.7 Candidate Genes for Allelic Association
- 1.8 Significance Levels
- 1.9 Meta-Analysis
- 1.10 Informatics
- 1.11 Summary
- References
- 2 Human DNA Sampling and Banking
- 2.1 Introduction
- 2.2 A Brief History of DNA Research
- 2.3 Goals and Needs of Human Population Genetics
- 2.4 Selection of the Source Tissue
- 2.5 A Fundamental Change in Sampling Methodology
- 2.6 DNA Isolation and Purification
- 2.7 Sample Storing
- 2.8 Sample Banking
- References
- 3 Microsatellite Genotyping
- 3.1 Introduction
- 3.2 Microsatellite Markers
- 3.3 The Genotyping Process - Experimental Considerations
- 3.4 Data Analysis
- 3.5 Data Management
- 3.6 Hardware