A Laboratory Guide for Cellular and Molecular Plant Biology

This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in o...

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Bibliographic Details
Main Authors: Negrutiu, I., Gharti (Author), Chhetri (Author)
Format: eBook
Published: Basel Birkhäuser 1991, 1991
Edition:1st ed. 1991
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Foreword
  • 1 Cellular Techniques — General Introduction
  • 1.1 Isolation and Culture of Protoplasts
  • 1.2 Mutagenic Treatments on Isolated Cells
  • 1.3 Somatic Hybridization
  • 1.4 Dilution Series as a Tool to Improve Medium Composition
  • 1.5 Plating Efficiency Evaluation in a Peroxidase Assay
  • 1.6 Production of Haploid Plants
  • 1.7 Isolation of Viable Microspores and Immature Pollen Grains from Cereal Inflorescences
  • 1.8 Isolation of Viable Sperm Cells from Corn (Zea mays) Pollen Grains
  • 1.9 Embryo Rescue in Nicotiana plumbaginifolia
  • 1.10 Use of Iodide Ions for Chemical Reduction of the Oxidative Agent H2O2 and Hypochlorites after Application as Decontaminating Agents for Plant Tissues
  • 2 Transformation Techniques — General Introduction
  • 2.1 Agrobacterium Transformation of Various Arabidopsis Expiants
  • 2.2 Direct Gene Transfer
  • 3 Extraction Techniques — General Introduction
  • 3.1 Isolation of DNA and RNA from Arabidopsis thaliana
  • 4.6 Preparation of Nuclear Extracts, Gel-Retardation Assay and DNAase I Footprinting
  • 4.7 Pulsed-Field Gel Electrophoresis of Plant DNA
  • 4.8 Assessing Methylation of Inserted DNA by Restriction with Isoschizomeric Enzymes and Inducing Demethylation with 5-Azacytidine
  • 5 Cytological Techniques — General Introduction
  • 5.1 Karyotyping with Protoplast Procedures
  • 5.2 In situ Hybridization
  • 5.3 How Cytometry of Nuclei for Ploidy and Cell Cycle Analysis
  • 5.4 Control of Cell Cycle Progression
  • 5.5 Induction and Isolation of Micronuclei and Microprotoplasts
  • 6 Appendices
  • 6.1 Culture Media and Basic Stock Solution
  • 6.4 Extraction and Purification of IAA and ABA
  • 7 Subject Index
  • 3.2 Total DNA Extraction — Alternative Protocols
  • 3.3 Characterization of Mitochondrial-DNA from Minute Quantities of Plant Material
  • 3.4 Generation of Large Amounts of cDNA by Polymerase Chain Reaction from Small Amounts of Total RNA
  • 3.5 Isolation of Nuclei from Plant Tissues
  • 3.6 Extraction of Amino Acids from Plant Samples and Their Analysis Using Ion-Exchange Chromatography
  • 3.7 Electroelution of Proteins from Plant Tissues
  • 3.8 Extraction, Purification and Analysis of Endogenous Indoleacetic Acid and Abscisic Acid
  • 4 Aspects of Structural and Functional Analysis of Genomes and Genes — General Introduction
  • 4.1 Southern Blot Analysis of Transgenic Nicotiana sp
  • 4.2 Northern Blot Analysis of ADH (Alcohol Dehydrogenase) Mutants in Arabidopsis
  • 4.3 Western Blot Detection of Proteins Synthesized Transiently inTransfected Plant Protoplasts
  • 4.4 Cloning Nuclear Single Copy Sequences for RFLP Analysis
  • 4.5 Run-on Transcription in Isolated Plant Nuclei