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Principles of Fluorescence Spectroscopy

`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expa...

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Bibliographic Details
Other Authors: Lakowicz, Joseph R. (Editor)
Format: eBook
Language:English
Published: New York, NY Springer US 1999, 1999
Edition:2nd ed. 1999
Subjects:
Physical Chemistry
Analytical Chemistry
Biophysics
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • 1. Introduction to Fluorescence
  • 2. Instrumentation for Fluorescence Spectroscopy
  • 3. Fluorophores
  • 4. Time-Domain Lifetime Measurements
  • 5. Frequency-Domain Lifetime Measurements
  • 6. Solvent Effects on Emission Spectra
  • 7. Dynamics of Solvent and Spectral Relaxation
  • 8. Quenching of Fluorescence
  • 9. Advanced Topics in Fluorescence Quenching
  • 10. Fluorescence Anisotropy
  • 11. Time-Dependent Anisotropy Decays
  • 12. Advanced Anisotropy Concepts
  • 13. Energy Transfer
  • 14. Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers
  • 15. Energy Transfer to Multiple Acceptors, in One, Two, or Three Dimensions
  • 16. Protein Fluorescence
  • 17. Time-Resolved Protein Fluorescence
  • 18. Excited-State Reactions
  • 19. Fluorescence Sensing
  • 20. Long-Lifetime Metal-Ligand Complexes
  • 21. DNA Technology
  • 22. Phase-Sensitive and Phase-Resolved Emission Spectra
  • Appendix I. Corrected Emission Spectra
  • 14. Latest Applications of Fluorescence
  • 15. Infrared and NIR Fluorescence
  • 16. Lasers
  • 17. Fluorescence Microscopy
  • 18. Metal-Ligand Complexes and Unusual Luminophores
  • Answers to Problems
  • 1. ?-Carboline Derivatives as Fluorescence Standards
  • 2. Corrected Emission Spectra of 9,10-Diphenylanthracene, Quinine Sulfate, and Fluorescein
  • 3. Long-Wavelength Standards
  • 4. Ultraviolet Standards
  • 5. Additional Corrected Emission Spectra
  • References
  • Appendix II. Fluorescent Lifetime Standards
  • 1. Nanosecond Lifetime Standards
  • 2. Picosecond Lifetime Standards
  • 3. Representative Frequency-Domain Intensity Decays
  • 4. Time-Domain Lifetime Standards
  • References
  • Appendix III. Additional Reading
  • 1. Lifetime Measurements
  • 2. Which Molecules are Fluorescent Representative Emission Spectra, and Practical Advice
  • 3. Theory of Fluorescence and Photophysics
  • 4. Principles of Fluorescence Spectroscopy
  • 5. Biochemical Fluorescence
  • 6. Protein Fluorescence
  • 7. Data Analysis and Nonlinear Least Squares
  • 8. Photochemistry
  • 9. Flow Cytometry.-10. Phosphorescence
  • 11. Polymer Science
  • 12. Fluorescence Sensing
  • 13. Immunoassays

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