Protocols for Neural Cell Culture

This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Cana...

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Bibliographic Details
Other Authors: Fedoroff, Sergey (Editor), Richardson, Arleen (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 1997, 1997
Edition:2nd ed. 1997
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • One Outgrowth Assays and Cortical Slice Cultures
  • Two Microexplant Cultures of the Cerebellum
  • Three Aggregating Neural Cell Cultures
  • Four Primary Cell Cultures of Peripheral and Central Neurons and Glia
  • Five Chick Spinal Somatic Motoneurons in Culture
  • Six Neural Cells in Microcultures: In Vitro Biological Assays for Neuroactive Agents
  • Seven Construction and Use of Compartmented Cultures
  • Eight Astrocyte and Oligodendrocyte Cultures
  • Nine Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium
  • Ten Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium
  • Eleven Culture of Glial Cells from Human Brain Biopsies
  • Twelve Colony Cultures
  • Thirteen Herpes Simplex Virus Vectors for Gene Therapy of the Nervous System
  • Fourteen Immunostaining and Identification of Antigens
  • Fifteen Elimination of Cell Types from Mixed Neural Cell Cultures
  • Sixteen Quantification of Cells in Culture
  • Seventeen Procedures for Subculturing Cells
  • Eighteen Preparation of Tissue-Cultured Material for Electron Microscopical Observation
  • Nineteen Tips in Tissue Culture
  • Twenty Use of Dissecting Instruments in Tissue Culture
  • Abbreviations and Definitions