Methods of Hybridoma Formation

Laymen often consider modern laboratory research to be based on an endless array of sophisticated technologies whose complex capabilities are as important to the outcome of any project as the inventiveness and creativity of the scientists who employ them. Scientists at times may share this point of...

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Corporate Author: SpringerLink (Online service)
Other Authors: Bartal, Arie H. (Editor), Hirshaut, Yashar (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1987, 1987
Edition:1st ed. 1987
Series:Contemporary Biomedicine
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Bystanders or Active Participants?
  • Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas
  • Chemically Mediated Cell Fusion
  • Electrofusion of Cells
  • Enhancement of Hybridoma Formation
  • Modification of HAT Medium and Hybridoma Formation
  • Culture Methods for the Selection and Isolation of Stable Antibody-Producing Murine Hybridomas
  • Selection of Growth Factors and Myelomas To Enhance Monoclonal Antibody-Producing Hybridoma Formation
  • Proliferation and Immune Secretion of B-Cell Hybridomas
  • Statistical Assessment of Hybridoma Monoclonality After Subcloning by the Limiting Dilution Technique
  • In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium
  • Intrasplenic Immunization for the Production of Monoclonal Antibodies
  • Use of Heteromyelomas in the Enhancement of Human Monoclonal Antibody Production
  • Human Hybridomas: Fusion Partners
  • An