Methods of Hybridoma Formation

Laymen often consider modern laboratory research to be based on an endless array of sophisticated technologies whose complex capabilities are as important to the outcome of any project as the inventiveness and creativity of the scientists who employ them. Scientists at times may share this point of...

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Bibliographic Details
Other Authors: Bartal, Arie H. (Editor), Hirshaut, Yashar (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 1987, 1987
Edition:1st ed. 1987
Series:Contemporary Biomedicine
Subjects:
Online Access:
Collection: Springer Book Archives -2004 - Collection details see MPG.ReNa
Table of Contents:
  • Current Methodologies in Hybridoma Formation
  • Retroviruses and Hybridoma Formation: Bystanders or Active Participants?
  • Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas
  • Chemically Mediated Cell Fusion
  • Electrofusion of Cells
  • Enhancement of Hybridoma Formation
  • Modification of HAT Medium and Hybridoma Formation
  • Culture Methods for the Selection and Isolation of Stable Antibody-Producing Murine Hybridomas
  • Selection of Growth Factors and Myelomas To Enhance Monoclonal Antibody-Producing Hybridoma Formation
  • Proliferation and Immune Secretion of B-Cell Hybridomas
  • Statistical Assessment of Hybridoma Monoclonality After Subcloning by the Limiting Dilution Technique
  • In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium
  • Intrasplenic Immunization for the Production of Monoclonal Antibodies
  • Use of Heteromyelomas in the Enhancement of Human Monoclonal Antibody Production
  • Human Hybridomas: Fusion Partners
  • Antibody-Secreting Human-Human Hybridomas: Technical Progress, Generation Obstacles, and Applications
  • Interspecific Hybridomas
  • Rat-Rat Hybridoma Formation and Rat Monoclonal Antibodies
  • Generation of Mutant Monoclonal Antibodies
  • Ranking the Affinities of Monoclonal Antibodies
  • Intrasplenic Inoculation: A Method for Rescuing Hybridoma Clones
  • Cryopreservation in Hybridoma Production
  • Early and Late Freezing of Hybridoma Clones
  • Flow Cytometry in Hybridoma Technology
  • Regulatory Issues Surrounding Therapeutic Use of Monoclonal Antibodies: Points To Consider in the Manufacture of Injectable Products Intended for Human Use
  • Human-Mouse Hybridoma Formation