Animal Cell Biotechnology Methods and Protocols
Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. Th...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2014, 2014
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| Edition: | 3rd ed. 2014 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- High-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter
- Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology
- Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine
- Scalable Transient Protein Expression
- DNA Profiling and Characterisation of Animal Cell Lines
- Tools for High-Throughput Process and Medium Optimization
- Designing Media for Animal Cell Culture – CHO Cells, The Industrial Standard
- Feed Optimization in Fed-Batch Culture
- Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches
- Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins
- Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System
- Monitoring Cell Growth, Viability and Apoptosis
- Metabolic Flux Estimation in Mammalian Cell Cultures
- Quenching Methods for the Analysis of Intracellular Metabolites
- NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors
- 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells.-Microfluidic Perfusion Culture
- Disposable Bioreactors for Inoculum Production and Protein Expression
- Configuration of Bioreactors
- Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes – Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM)
- Cell Assessment by Atline Microscopy
- Seed Train Optimization for Cell Culture
- Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines
- Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles
- Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology
- Virus Separation Using Membranes