Plant Cell Morphogenesis Methods and Protocols

 Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis. Methods and techniques include contemporary takes on  classical light microscopy  and  histochemistry through automated microscop...

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Bibliographic Details
Other Authors: Žárský, Viktor (Editor), Cvrčková, Fatima (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2014, 2014
Edition:1st ed. 2014
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Essential Methods of Plant Sample Preparation for Light Microscopy
  • Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
  • Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues
  • Automated Microscopy in Forward Genetic Screening of Arabidopsis
  • Image Analysis: Basic Procedures for Description of Plant Structures on Anatomical Sections
  • Identifying Subcellular Protein Localization with Fluorescent Protein Fusions After Transient Expression in Onion Epidermal Cells
  • Visualizing and Quantifying the in vivo Structure and Dynamics of the Arabidopsis Cortical Cytoskeleton using CLSM and VAEM
  • Sequential Replicas for In Vivo Imaging of Growing Organ Surfaces
  • Time-lapse Imaging of Developing Meristems using Confocal Laser Scanning Microscope
  • Quantifying Cell Shape and Gene Expression in the Shoot Apical Meristem using MorphoGraphX
  • Mechanical Measurements on Living Plant Cells by Micro-indentation with Cellular Force Microscopy
  • High-pressure Freezing and Low-temperature Processing of Plant Tissue Samples for Electron Microscopy
  • Reconstructing Plant Cells in 3D by Serial Section Electron Tomography
  • Imaging Plant Nuclei and Membrane-associated Cytoskeleton by Field Emission Scanning Electron Microscopy
  • Immuno-gold Labeling of Resin-embedded Electron Microscopical Sections
  • Live Cell Imaging of Arabidopsis Root Hairs
  • Morphological Analysis of Cell Growth Mutants in Physcomitrella
  • Plant Cell Lines in Cell Morphogenesis Research
  • Antisense Oligodeoxynucleotide-mediated Gene Knock-down in Pollen Tubes
  • Lab-on-a-Chip for Studying Growing Pollen Tubes
  • Laser Microdissection of Plant Cells
  • Optical Trapping in Plant Cells
  • Heterologous Expression in Budding Yeast as a Tool for Studying the Plant Cell Morphogenesis Machinery