Ion Channels Methods and Protocols

Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie...

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Bibliographic Details
Other Authors: Gamper, Nikita (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2013, 2013
Edition:2nd ed. 2013
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Approaches to Cloning of Pain-Related Ion Channel Genes
  • Mammalian Expression Systems and Transfection Techniques
  • Use of Escherichia coli for the Production and Purification of Membrane Proteins
  • Transient Overexpression of Genes in Neurons Using Nucleofection
  • Viral Gene Delivery: Optimized Protocol for Production of High Titre Lentiviral Vectors
  • Two-Electrode Voltage Clamp
  • Conventional Micropipette-Based Patch Clamp Techniques
  • Recording of Ion Channel Activity in Planar Lipid Bilayers Experiments
  • Recording Macroscopic Currents in Large Patches from Xenopus Oocytes
  • Combined Single-Channel and Macroscopic Recording Techniques to Analyze Gating Mechanisms of the Large Conductance Ca2+ and Voltage Activated (BK) Potassium Channel
  • Perforated Whole Cell Patch Clamp Recording
  • Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons
  • Automated Planar Patch-Clamp
  • Recording Single-Channel Currents Using “Smart Patch-Clamp” Technique
  • Studying Ion Channels in Human Erythrocytes by Direct and Indirect Means
  • Recording Ion Channels in Isolated, Split-Opened Tubules
  • Single Channel Analysis of TRPC Channels in the Podocytes of Freshly Isolated Glomeruli
  • Ca2+ Imaging as a Tool to Assess TRP Channel Function in Murine Distal Nephrons
  • Patch Clamping Drosophila Sensory Neurons
  • Production and Validation of Recombinant Adeno-Associated Virus for Channelrhodopsin Expression in Neurons
  • Optical Control of Ligand-Gated Ion Channels
  • Using Total Internal Reflection Fluorescence Microscopy to Observe Ion channel Trafficking and Assembly
  • Förster Resonance Energy Transfer-Based Imaging at the Cell Surface of Live Cells
  • The Use of Dansyl-Calmodulin to Study Interactions with Channels and Other Proteins
  • Imaging and Quantification of Recycled KATP Channels
  • Generation of Antibodies that Are Externally-Acting Isoform-Specific Inhibitors of Ion Channels
  • Site-Directed Mutagenesis to Study the Structure-Function Relationships of Ion Channels
  • Cysteine-Based Cross-Linking Approach to Study Inter-Domain Interactions in Ion Channel Functions
  • Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-RayCrystallography
  • Isotope Labeling Strategies for Analysis of an Ion Channel Cytoplasmic Domain by NMR Spectroscopy
  • Recording Dendritic Ion Channel Properties and Function from Cortical Neurons
  • M Current Recording from Acute DRG Slices