Plant Signalling Networks Methods and Protocols

Signal transduction is the fundamental mechanism for regulation of cellular activities by environmental cues and regulatory signals, and is particularly important for plants, whose survival requires proper physiological and developmental responses to the environmental changes. Much progress has been...

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Bibliographic Details
Other Authors: Wang, Zhi-Yong (Editor), Yang, Zhenbiao (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2012, 2012
Edition:1st ed. 2012
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Experimental Analysis of Receptor Kinase Phosphorylation
  • Quantitative Measurement of Phosphopeptides and Proteins via Stable Isotope Labeling in Arabidopsis (SILIA) and Functional Phosphoproteomic Strategies
  • Identification of O-GlcNAc Modified Proteins from Arabidopsis
  • Quantitative Analysis of Protein Phosphorylation Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
  • Quantitative Analysis of Plasma Proteome Using Two-Dimensional Difference Gel Electrophoresis (2-D DIGE)
  • Identification and Verification of Redox-Sensitive Proteins in Arabidopsis thaliana
  • Small-Molecule Dissection of Brassinosteroid (BR) Signaling
  • A Chemical Genetics Method to Uncover Small Molecules for Dissecting the Mechanism of ABA Responses in Arabidopsis Seed Germination
  • Activation Tagging
  • Rho GTPase Activity Analysis in Plant Cells
  • Analysis of In Vivo ROP GTPase Activity at the Subcellular Level by Fluorescence Resonance Energy Transfer Microscopy
  • In vivo Ubiquitination Assay by Agroinfiltration
  • In Vitro Protein Ubiquitination Assay
  • Genome-Wide Identification of Transcription Factor Binding Sites in Plants Using Chromatin Immunoprecipitation Followed by Microarray (ChIP-chip) or Sequencing (ChIP-seq)
  • Smart Pooling of mRNA Samples for Efficient Transcript Profiling
  • Transient Expression Assays for Quantifying Signaling Output
  • Genome-Wide Profiling of Uncapped mRNA
  • Computational Tools for Quantitative Analysis of Cell Growth Patterns and Morphogenesis in Actively Developing Plant Stem Cell Niches