Host-Fungus Interactions Methods and Protocols

Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.  Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this...

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Bibliographic Details
Other Authors: Brand, Alexandra C. (Editor), MacCallum, Donna M. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2012, 2012
Edition:1st ed. 2012
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization
  • A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections
  • Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter
  • Orogastrointestinal Model of Mucosal and Disseminated Candidiasis
  • A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis
  • In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus
  • Biofilm Formation Studies in Microtiter Plate Format
  • Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of Infection
  • Laser Capture Microdissection of Candida albicans from Host Tissue
  • Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples
  • Cytokine Measurement Using Cytometric Bead Arrays (CBA)
  • Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis
  • Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi
  • Drosophila melanogaster as a Model Organism for Invasive Aspergillosis
  • Galleria mellonella as a Model for Fungal Pathogenicity Testing
  • Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi
  • Mouse Intravenous Challenge Models and Applications
  • A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis
  • Invasive Models of Histoplasmosis
  • Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA
  • Tetracycline-Inducible Gene Expression in Candida albicans
  • Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii
  • Modular Gene Over-Expression Strategies for Candida albicans
  • Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans
  • Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans
  • Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils
  • Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions
  • Simple Assays for Measuring Innate Interactions with Fungi
  • Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells.-Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium
  • Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR
  • Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy
  • Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans
  • Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans
  • Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi
  • Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers
  • Multiple Gene Deletion in Cryptococcus neoformans Using the Cre
  • lox System
  • Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast
  • Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach
  • Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers
  • RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum
  • RNA Interference in Cryptococcus neoformans