Allostery : Methods and Protocols

Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/functio...

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Corporate Author: SpringerLink (Online service)
Other Authors: Fenton, Aron W. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2012, 2012
Edition:1st ed. 2012
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Binding Techniques to Study the Allosteric Energy Cycle
  • Kinetic Trapping of a Key Hemoglobin Intermediate
  • Allosteric Coupling Between Transition Metal Binding Sites in Homooligomeric Metal Sensor Proteins
  • Studying the Allosteric Energy Cycle by Isothermal Titration Calorimetry
  • Detecting “Silent” Allosteric Coupling
  • Using Mutant Cycle Analysis to Elucidate Long-Range Functional Coupling in Allosteric Receptors
  • A Review of Methods Used for Identifying Structural Changes in a Large Protein Complex
  • Allosteric Mechanisms of G Protein-Coupled Receptor Signaling: A Structural Perspective
  • Dynamic Light Scattering to Study Allosteric Regulation
  • Dissecting the Linkage Between Transcription Factor Self-Assembly and Site-Specific DNA Binding: The Role of the Analytical Ultracentrifuge
  • Fluorescence Correlation Spectroscopy and Allostery: The Case of GroEL
  • The Morpheein Model of Allostery: Evaluating Proteins as Potential Morpheeins
  • Combining NMR and Molecular Dynamics Studies for Insights into the Allostery of Small GTPase-Protein Interactions
  • H/D-Exchange Study of an Allosteric Energy Cycle
  • Ensemble Properties of Network Rigidity Reveal Allosteric Mechanisms
  • An In Vivo Approach to Isolating Allosteric Pathways Using Hybrid Multimeric Proteins
  • Mutations in the GABAA Receptor that Mimic the Allosteric Ligand, Etomidate
  • Allosteric Regulation of Human Liver Pyruvate Kinase by Peptides that Mimic the Phosphorylated/Dephosphorylated N-Terminus
  • In Silico Screening Approaches for Lead Generation: Identification of Novel Allosteric Modulators of Human-Erythrocyte Pyruvate Kinase
  • Identification of Allosteric-Activating Drug Leads for Human Liver Pyruvate Kinase
  • A Critical Evaluation of Correlated Mutation Algorithms and Coevolution Within Allosteric Mechanisms
  • The Advantage of Global Fitting of Data Involving Complex Linked Reactions
  • Predicting Binding Sites by Analyzing Allosteric Effects