Plant Kinases Methods and Protocols
Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2011, 2011
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| Edition: | 1st ed. 2011 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Guide to the Book Plant Kinases
- The Age of Protein Kinases
- Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
- Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System
- Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants
- Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks
- Bacterial Assay to Study Plant Sensor Histidine Kinases.- Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase
- Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen
- Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules
- Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization
- Quantifying Degradation Rates of Trans-Membrane Receptor Kinases
- Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta
- Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo
- Chemical Genetic Analysis of Protein Kinase Function in Plants
- Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes
- Phosphoproteomics Using iTRAQ.