Molecular Methods for Evolutionary Genetics
We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overv...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2011, 2011
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| Edition: | 1st ed. 2011 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Identification of Homologous Gene Sequences by PCR with Degenerate Primers
- Characterizing cDNA Ends by Circular RACE
- Identification of DNA Sequences that Flank a Known Region by Inverse PCR
- Quantification of Transcript Levels with Quantitative RT-PCR
- Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences
- Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern
- Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning
- Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution
- Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution
- Making Reporter Gene Constructs to Analyze Cis-regulatory Elements
- PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker’s Yeast
- Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species Transgensis
- Recombination-mediated Genetic Engineering of Large Genomic DNA Transgenes
- Overlap Extension PCR: An Efficient Method for Transgene Construction
- Gene Knockdown Analysis by Double-stranded RNA Injection
- Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei
- Chromosome Analysis in Invertebrates and Vertebrates
- Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries
- Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones
- Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing
- Accessing the Transcriptome: How to Normalize mRNA Pools
- Transcriptome Sequencing Goals, Assembly, and Assessment
- Rapid Retrieval of DNA Target Sequences by Primer Extension Capture
- SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing
- DNA Microarray-based Mutation Discovery and Genotyping
- Genotyping with Sequenom
- Isolating Microsatellite Loci: Looking Back, Looking Ahead
- Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector