High-Throughput Next Generation Sequencing Methods and Applications

Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities....

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Corporate Author: SpringerLink (Online service)
Other Authors: Kwon, Young Min (Editor), Ricke, Steven C. (Editor)
Format: eBook
Published: Totowa, NJ Humana Press 2011, 2011
Edition:1st ed. 2011
Series:Methods in Molecular Biology
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Helicos Single Molecule Sequencing of Bacterial Genomes
  • Whole Genome Sequencing of Unculturable Bacterium Using Whole Genome Amplification
  • RNA Sequencing and Quantitation Using the Helicos Genetic Analysis System
  • Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing
  • Discovery of Bacterial sRNAs by High-Throughput Sequencing
  • Identification of Virus Encoding MicroRNAs Using 454 FLX Sequencing Platform
  • Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Applications
  • Integrating High-Throughput Pyrosequencing and Quantitative Real-Time PCR to Analyze Complex Microbial Communities
  • Tag-Encoded FLX Amplicon Pyrosequencing for the Elucidation of Microbial and Functional Gene Diversity in Any Environment
  • Pyrosequencing of Chaperonin-60 (cpn60) Amplicons as a Means of Determining Microbial Community Composition
  • Prescreening of Microbial Populations for the Assessment of Sequencing Potential
  • Metagenomics
  • Metagenomic Analysis