Plant Developmental Biology Methods and Protocols
The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In P...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2010, 2010
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| Edition: | 1st ed. 2010 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Growth Protocols for Model Plants in Developmental Biology
- Grafting as a Research Tool
- Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function
- The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level
- Inducible Gene Expression Systems for Plants
- Trichome Development in Arabidopsis
- Phenotyping the Development of Leaf Area in Arabidopsis thaliana
- Analyzing Shoot Apical Meristem Development
- Analyzing Floral Meristem Development
- Female Gametophytic Mutants: Diagnosis and Characterization
- Pollen Tube Development
- Analysis of Root Meristem Size Development
- Phenotypic Characterization of Photomorphogenic Responses During Plant Development
- Kinematic Analysis of Cell Division and Expansion
- Flowering Time Control
- mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis
- Immunolocalization of Proteins in Plants
- Detection of Small Non-coding RNAs
- Quantitative Real Time PCR in Plant Developmental Biology
- Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics
- Fluorescence-Activated Cell Sorting in Plant Developmental Biology
- Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling
- Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay Protein–Protein Interaction in Plants
- The Split Luciferase Complementation Assay
- Co-immunoprecipitation and Protein Blots
- Probing Protein–Protein Interactions with FRET–FLIM
- Plant Chromatin Immunoprecipitation
- Immunocytological Analysis of Chromatin in Isolated Nuclei
- Bisulphite Sequencing of Plant Genomic DNA.