Heterologous Expression of Membrane Proteins Methods and Protocols
Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer’s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. In Heterologous Expression of Membrane Proteins:...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2010, 2010
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| Edition: | 1st ed. 2010 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Heterologous Expression of Membrane Proteins for Structural Analysis
- Production of Membrane Proteins in Escherichia coli and Lactococcus lactis
- Mammalian Membrane Receptors Expression as Inclusion Bodies in Escherichia coli
- Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies
- Membrane Protein Expression in Lactococcus lactis
- Heterologous Expression of Human Membrane Receptors in the Yeast Saccharomyces cerevisiae
- Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells
- Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model
- Membrane Protein Expression in the Eyes of Transgenic Flies
- Expression of Mammalian Membrane Proteins in Mammalian Cells Using Semliki Forest Virus Vectors
- Membrane Protein Expression in Cell-Free Systems
- Practical Considerations of Membrane Protein Instability during Purification and Crystallisation
- Membrane Protein Solubilization
- Amphipols and Fluorinated Surfactants: Two Alternatives to Detergents for Studying Membrane Proteins In vitro
- Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sarcoplasmic Reticulum Ca2+-ATPase