Antibody Phage Display Methods and Protocols
Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Seco...
| Other Authors: | |
|---|---|
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2009, 2009
|
| Edition: | 2nd ed. 2009 |
| Series: | Methods in Molecular Biology
|
| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Antibody Phage Display: Overview of a Powerful Technology that Has Quickly Translated to the Clinic
- Design and Construction of Synthetic Phage-Displayed Fab Libraries
- Affinity Isolation of Antigen-Specific Circulating B Cells for Generation of Phage Display-Derived Human Monoclonal Antibodies
- Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors
- Anti-?2GP-I and Anti-Prothrombin Antibodies Generated by Phage Display
- The Generation and Selection of Single-Domain, V Region Libraries from Nurse Sharks
- The Isolation of scFvs Against Small Target Molecules
- Display and Selection of scFv Antibodies on HEK-293T Cells
- Isolation of scFvs that Inhibit the NS3 Protease of Hepatitis C Virus by a Combination of Phage Display and a Bacterial Genetic Screen
- Guided Selection Methods Through Chain Shuffling
- Sequential Antigen Panning for Selection of Broadly Cross-Reactive HIV-1-Neutralizing Human Monoclonal Antibodies
- Selection of Antibodies Able to Rapidly Enter Mammalian Cells
- Identification of the Specificity of Isolated Phage Display Single-Chain Antibodies Using Yeast Two-Hybrid Screens
- Generation of Bispecific and Tandem Diabodies
- Intrabody Expression in Eukaryotic Cells
- High-Level Periplasmic Expression and Purificationof scFvs
- Expression of Single-Chain Fv Fragments in E. coli Cytoplasm
- High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris