Antibiotic Resistance Protocols Second Edition

Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and...

Full description

Bibliographic Details
Other Authors: Gillespie, Stephen H. (Editor), McHugh, Timothy D. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2010, 2010
Edition:2nd ed. 2010
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Epidemiology and Population Genetics
  • The RM Test for Determining Methicillin-Resistant Staphylococcus aureus Lineages
  • Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx
  • Genomics and Gene Expression
  • Methods to Determine Antibiotic Resistance Gene Silencing
  • Studying Gene Induction of Glycopeptide Resistance Using Gene Swapping
  • Real-Time PCR Methods to Study Expression of Genes Related to Hypermutability
  • Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial Compounds
  • Fitness Mutation and Physiology
  • Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity
  • Measurements of Heme Levels and Respiration Rate in Salmonella enterica Serovar Typhimurium
  • Methods to Determine Fitness in Bacteria
  • Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosis
  • Detection of Resistance
  • Identification of Efflux-Mediated Multi-drug Resistance in Bacterial Clinical Isolates by Two Simple Methods
  • Evaluation of Efflux Activity of Bacteria by a Semi-automated Fluorometric System
  • Measuring the Activity of Active Efflux in Gram-Negative Bacteria
  • Rapid Characterization of ?-Lactamases by Multiplex PCR
  • Rapid Methods for Testing Inhibitors of Mycobacterial Growth
  • Use of Biofilm Model Systems to Study Antimicrobial Susceptibility
  • Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing®