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Bacteriophages Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions

Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowled...

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Bibliographic Details
Other Authors: Clokie, Martha R. J. (Editor), Kropinski, Andrew (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2009, 2009
Edition:1st ed. 2009
Series:Methods in Molecular Biology
Subjects:
Bacteria
Microbiology
Medicine / Research
Biology / Research
Biochemistry
Biomedical Research
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Bacteriophage Isolation
  • Methods for the Isolation of Viruses from Environmental Samples
  • Bacteriophage Enrichment from Water and Soil
  • Isolation of Phage via Induction of Lysogens
  • Isolation of Cyanophages from Aquatic Environments
  • Isolation of Viruses from High Temperature Environments
  • Isolation of Novel Large and Aggregating Bacteriophages
  • Bacteriophage Characterization
  • Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay
  • Enumeration of Bacteriophages by the Direct Plating Plaque Assay
  • Enumeration of Bacteriophages Using the Small Drop Plaque Assay System
  • Determination of Virus Abundance by Epifluorescence Microscopy
  • Enumeration of Bacteriophages Using Flow Cytometry
  • Basic Phage Electron Microscopy
  • Phage Classification and Characterization
  • Phage Host Range and Efficiency of Plating
  • Measurement of the Rate of Attachment of Bacteriophage to Cells
  • Measurement of the Bacteriophage Inactivation Kinetics with Purified Receptors
  • Bacteriophage Plaques: Theory and Analysis
  • Practical Methods for Determining Phage Growth Parameters
  • Phage Production and Maintenance of Stocks, Including Expected Stock Lifetimes
  • Bacteriophage-Host Interactions
  • Construction of Phage Mutants
  • Modifying Bacteriophage with Recombineering
  • Identification and Isolation of Lysogens with Induced Prophage
  • Generalized Transduction
  • Preparation and Characterization of Anti-phage Serum
  • Generalized Transduction by Lytic Bacteriophages

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