Pichia Protocols

The use of Pichia Pastoris as a recombinant protein system has increased greatly in popularity over the years. In Pichia Protocols 2nd Edition, the author focuses on new information that has come to light in recent years but repeats key basic methods so that researchers are not absolutely dependent...

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Bibliographic Details
Other Authors: Cregg, James M. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2007, 2007
Edition:2nd ed. 2007
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Vectors and Strains for Expression
  • DNA-Mediated Transformation
  • Rational Design and Optimization of Fed-Batch and Continuous Fermentations
  • Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1)
  • Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C
  • Rapid Screening of Chromatography Resins for the Purification of Proteins
  • Characterization of O-Linked Saccharides on Glycoproteins
  • Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids
  • N-Linked Glycan Characterization of Heterologous Proteins
  • Heavy Labeling of Recombinant Proteins
  • Selenomethionine Labeling of Recombinant Proteins
  • Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains
  • Classical Genetics
  • Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration
  • Characterization of Protein-Protein Interactions
  • Localization of Proteins and Organelles Using Fluorescence Microscopy
  • Fluorescence Microscopy and Thin-Section Electron Microscopy