Macrophages and Dendritic Cells Methods and Protocols
In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers...
Other Authors: | |
---|---|
Format: | eBook |
Language: | English |
Published: |
Totowa, NJ
Humana Press
2009, 2009
|
Edition: | 1st ed. 2009 |
Series: | Methods in Molecular Biology
|
Subjects: | |
Online Access: | |
Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors
- Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions
- Macrophage Activation: Classical Vs. Alternative
- Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy
- Detection of Activated Rab7 GTPase with an Immobilized RILP Probe
- Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution
- Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors
- Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor
- The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities
- Genetic Modification of Murine Dendritic Cells by RNA Transfection
- Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities
- Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages
- Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology
- Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages
- Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice
- Analysis of Matrix Metalloproteinase Secretion by Macrophages
- Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets
- Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines
- Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages
- Large-Scale Phagosome Preparation
- Coordinate Regulation of Sphingosine Kinase and Actin Dynamics