Macrophages and Dendritic Cells Methods and Protocols

In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers...

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Bibliographic Details
Other Authors: Reiner, Neil E. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2009, 2009
Edition:1st ed. 2009
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors
  • Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions
  • Macrophage Activation: Classical Vs. Alternative
  • Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy
  • Detection of Activated Rab7 GTPase with an Immobilized RILP Probe
  • Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution
  • Multivalent Protein Probes for the Identification and Characterization of Cognate Cellular Ligands for Myeloid Cell Surface Receptors
  • Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor
  • The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities
  • Genetic Modification of Murine Dendritic Cells by RNA Transfection
  • Recording Phagosome Maturation Through the Real-Time, Spectrofluorometric Measurement of Hydrolytic Activities
  • Derivation and Characterization of Murine Alternatively Activated (M2) Macrophages
  • Isolation and Culture of Human Hematopoietic Progenitors for Studies of Dendritic Cell Biology
  • Generation, Culture and Flow-Cytometric Characterization of Primary Mouse Macrophages
  • Analyzing Classical and Alternative Macrophage Activation in Macrophage/Neutrophil-Specific IL-4 Receptor-Alpha-Deficient Mice
  • Analysis of Matrix Metalloproteinase Secretion by Macrophages
  • Identification of Pathogen Receptors on Dendritic Cells to Understand their Function and to Identify New Drug Targets
  • Stable Lentiviral Vector-Mediated Gene Silencing in Human Monocytic Cell Lines
  • Use of Fluorescent Probes to Detect Lipid Signaling Intermediates in Macrophages
  • Large-Scale Phagosome Preparation
  • Coordinate Regulation of Sphingosine Kinase and Actin Dynamics