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130626 ||| eng |
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|a 9781597453639
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| 100 |
1 |
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|a Baumann, Peter
|e [editor]
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| 245 |
0 |
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|a G-Quadruplex DNA
|h Elektronische Ressource
|b Methods and Protocols
|c edited by Peter Baumann
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| 250 |
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|a 1st ed. 2010
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| 260 |
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|a Totowa, NJ
|b Humana
|c 2010, 2010
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| 300 |
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|a XII, 276 p
|b online resource
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| 505 |
0 |
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|a G-Quadruplexes: From Guanine Gels to Chemotherapeutics -- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes -- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential -- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis -- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay -- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy -- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures -- 2-Aminopurine as a Probe for Quadruplex Loop Structures -- Assessing DNA Structures with 125I Radioprobing -- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques -- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments -- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC -- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography -- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes -- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands
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| 653 |
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|a Cell Biology
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| 653 |
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|a Medical Genetics
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| 653 |
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|a Cytology
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| 653 |
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|a Medical genetics
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| 653 |
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|a Biochemistry
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| 041 |
0 |
7 |
|a eng
|2 ISO 639-2
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| 989 |
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|b Springer
|a Springer eBooks 2005-
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| 490 |
0 |
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|a Methods in Molecular Biology
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| 028 |
5 |
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|a 10.1007/978-1-59745-363-9
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| 856 |
4 |
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|u https://doi.org/10.1007/978-1-59745-363-9?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572
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| 520 |
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|a Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.
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