High Throughput Protein Expression and Purification Methods and Protocols
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in us...
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| Format: | eBook |
| Language: | English |
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Totowa, NJ
Humana
2009, 2009
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| Edition: | 1st ed. 2009 |
| Series: | Methods in Molecular Biology
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| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- High-Throughput Protein Production (HTPP): A Review of Enabling Technologies to Expedite Protein Production
- Designing Experiments for High-Throughput Protein Expression
- Gateway Cloning for Protein Expression
- Flexi Vector Cloning
- The Precise Engineering of Expression Vectors Using High-Throughput In-Fusion™ PCR Cloning
- The Polymerase Incomplete Primer Extension (PIPE) Method Applied to High-Throughput Cloning and Site-Directed Mutagenesis
- A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
- “System 48” High-Throughput Cloning and Protein Expression Analysis
- Automated 96-Well Purification of Hexahistidine-Tagged Recombinant Proteins on MagneHis Ni2+-Particles
- E. coli and Insect Cell Expression, Automated Purification and Quantitative Analysis
- Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner for the Production of Soluble Recombinant Proteins in Escherichia coli
- PHB-Intein-Mediated Protein Purification Strategy
- High-Throughput Biotinylation of Proteins
- High-Throughput Insect Cell Protein Expression Applications
- High-Throughput Protein Expression Using Cell-Free System
- The Production of Glycoproteins by Transient Expression in Mammalian Cells
- High-Throughput Expression and Detergent Screening of Integral Membrane Proteins
- Cell-Free Expression for Nanolipoprotein Particles: Building a High-Throughput Membrane Protein Solubility Platform
- Expression and Purification of Soluble His6-Tagged TEV Protease
- High-Throughput Protein Concentration and Buffer Exchange: Comparison of Ultrafiltration and Ammonium Sulfate Precipitation