Protein Engineering Protocols
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evol...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2007, 2007
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| Edition: | 1st ed. 2007 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Design and Computational Strategies for Protein Engineering
- Combinatorial Protein Design Strategies Using Computational Methods
- Global Incorporation of Unnatural Amino Acids in Escherichia coli
- Considerations in the Design and Optimization of Coiled Coil Structures
- Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions
- Design and Synthesis of Artificial Zinc Finger Proteins
- Monobodies
- Engineering Site-Specific Endonucleases
- Evolutionary Strategies for Protein Engineering
- Protein Library Design and Screening
- Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids
- Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology
- Degenerate Oligonucleotide Gene Shuffling
- M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display
- Ribosome-Inactivation Display System
- Compartmentalized Self-Replication
- Synthesis of Degenerated Libraries of the Ras-Binding Domain of Raf and Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay
- A General Method of Terminal Truncation, Evolution, and Re-Elongation to Generate Enzymes of Enhanced Stability