SARS- and Other Coronaviruses : Laboratory Protocols

In 2003, the word "coronavirus" spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subje...

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Corporate Author: SpringerLink (Online service)
Other Authors: Cavanagh, Dave (Editor)
Format: eBook
Published: Totowa, NJ Humana Press 2008, 2008
Edition:1st ed. 2008
Series:Methods in Molecular Biology
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Detection and Discovery of Coronaviruses
  • A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
  • Detection of Group 1 Coronaviruses in Bats Using Universal Coronavirus Reverse Transcription Polymerase Chain Reactions
  • Detection and Sequence Characterization of the 3?-End of Coronavirus Genomes Harboring the Highly Conserved RNA Motif s2m
  • RT-PCR Detection of Avian Coronaviruses of Galliform Birds (Chicken, Turkey, Pheasant) and in a Parrot
  • Detection of Group 2a Coronaviruses with Emphasis on Bovine and Wild Ruminant Strains
  • Detection of SARS Coronavirus in Humans and Animals by Conventional and Quantitative (Real Time) Reverse Transcription Polymerase Chain Reactions
  • Detection of New Viruses by VIDISCA
  • Isolation, Growth, Titration, and Purification of Coronaviruses
  • Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
  • Generation of Recombinant Coronaviruses Using Vaccinia Virus as the Cloning Vector and Stable Cell Lines Containing Coronaviral Replicon RNAs
  • Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses
  • Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes
  • Systematic Assembly and Genetic Manipulation of the Mouse Hepatitis Virus A59 Genome
  • Identifying Coronavirus Receptors
  • Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes
  • Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins
  • The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration
  • Isolation and Propagation of Coronaviruses inEmbryonated Eggs
  • Large-Scale Preparation of UV-Inactivated SARS Coronavirus Virions for Vaccine Antigen
  • Structure of Covs Analyzed by Electron Microscopy
  • Purification and Electron Cryomicroscopy of Coronavirus Particles
  • Expression of Coronavirus Proteins and Crystallization
  • Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization
  • Raising Antibodies to Coronavirus Proteins
  • Generating Antibodies to the Gene 3 Proteins of Infectious Bronchitis Virus
  • Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
  • Production of Monospecific Rabbit Antisera Recognizing Nidovirus Proteins
  • Manipulating the Genomes of Coronaviruses
  • Manipulation of the Coronavirus Genome Using Targeted RNA Recombination with Interspecies Chimeric Coronaviruses