Nuclear Transfer Protocols Cell Reprogramming and Transgenesis

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The authors provide readily reproducible techniques for the generation of cloned...

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Bibliographic Details
Other Authors: Verma, Paul J. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana 2006, 2006
Edition:1st ed. 2006
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Nuclear Transfer
  • Origin and Progress of Nuclear Transfer in Nonmammalian Animals
  • In Vitro Maturation and Embryo Production in Cattle
  • Activation of Oocytes After Nuclear Transfer
  • Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos
  • Maintenance of Pregnancy in Pigs With Limited Viable Embryos
  • Health and Neonatal Care of Bovine Clones
  • Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice
  • Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
  • A Double Nuclear Transfer Technique for Cloning Pigs
  • Nuclear Transfer in Nonhuman Primates
  • Cloning of Exotic/Endangered Species: Desert Bighorn Sheep
  • Handmade Somatic Cell Cloning in Cattle
  • Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells
  • Transgenesis Using Nuclear Transfer in Goats
  • Reprogramming Nuclei
  • Nuclear Reprogramming
  • Expression of Imprinted Genes in Cloned Mice
  • Nuclear Remodeling Assay in Xenopus Egg Extract
  • In Vitro Reprogramming of Nuclei and Cells
  • Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells
  • Gene Expression and Methylation Patterns in Cloned Embryos
  • Concluding Remarks
  • Are There Any Normal Clones?
  • Future and Applications of Cloning