Organelle Proteomics
Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purifi...
| Other Authors: | , |
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ
Humana
2008, 2008
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| Edition: | 1st ed. 2008 |
| Series: | Methods in Molecular Biology
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| Subjects: | |
| Online Access: | |
| Collection: | Springer eBooks 2005- - Collection details see MPG.ReNa |
Table of Contents:
- Purification and Proteomic Analysis of Plant Plasma Membranes
- Protocol to Enrich and Analyze Plasma Membrane Proteins from Frozen Tissues
- Proteomic Characterization of Membrane Protein Topology
- Free Flow Isoelectric Focusing
- Use of Gas-Phase Fractionation to Increase Protein Identifications
- Purification and Proteomic Analysis of Lysosomal Integral Membrane Proteins
- Affinity Purification of Soluble Lysosomal Proteins for Mass Spectrometric Identification
- Purification and Proteomic Analysis of Synaptic Vesicles
- Purification and Proteomics Analysis of Pancreatic Zymogen Granule Membranes
- Isolation and Proteomic Analysis of Chlamydomonas Centrioles
- Purification and Proteomic Analysis of 20S Proteasomes from Human Cells
- Characterization of E. coli Ribosomal Particles
- Cellular, Genetic and Molecular Mechanisms of PlantEmbryogenesis
- Assessment of Organelle Purity Using Antibodies and Specific Assays
- Identifying Bona Fide Components of an Organelle by Isotope-Coded Labeling of Subcellular Fractions
- Determination of Genuine Residents of Plant Endomembrane Organelles using Isotope Tagging and Multivariate Statistics
- Quantitative Proteomic Analysis to Profile Dynamic Changes in the Spatial Distribution of Cellular Proteins
- Use of Transcriptomic Data to Support Organelle Proteomic Analysis
- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry
- Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry
- Analysis of Organelles, Vesicles, and Protein Machineries
- Purification and Proteomic Analysis of Chloroplasts and their Sub-Organellar Compartments
- Proteomic Analysis of the Stacked Golgi Complex
- Purification of Saccharomyces cerevisiae Mitochondria by Zone Electrophoresis in a Free Flow Device
- Preparation of Respiratory Chain Complexes from Saccharomyces cerevisiae Wild-Type and Mutant Mitochondria
- Mitochondrial Proteomics
- Purification and Proteomic Analysis of the Mouse Liver Mitochondrial Inner Membrane
- Subcellular Fractionation and Proteomics of Nuclear Envelopes
- Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
- Preparation Methods of Human Metaphase Chromosomes for their Proteome Analysis