Methods to Study Litter Decomposition A Practical Guide

Decomposition of organic matter is a major ecosystem process involving an array of different organisms, including bacteria, fungi and invertebrates. The main objective of this book is to provide students and laboratory instructors at universities and professional ecologists with a broad range of est...

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Bibliographic Details
Other Authors: Graça, Manuel A.S. (Editor), Bärlocher, Felix (Editor), Gessner, Mark O. (Editor)
Format: eBook
Language:English
Published: Dordrecht Springer Netherlands 2005, 2005
Edition:1st ed. 2005
Subjects:
Online Access:
Collection: Springer eBooks 2005- - Collection details see MPG.ReNa
Table of Contents:
  • Litter Dynamics
  • Litter Input
  • Leaf Retention
  • Manipulation of Stream Retentiveness
  • Coarse Benthic Organic Matter
  • Leaching
  • Leaf Mass Loss Estimated by Litter Bag Technique
  • Coarse Particulate Organic Matter Budgets
  • Leaf Chemical And Physical Properties
  • Determination of Total Nitrogen and Phosphorus in Leaf Litter
  • Total Protein
  • Free Amino Acids
  • Determination of Total Carbohydrates
  • Determination of Soluble Carbohydrates
  • Total Lipids
  • Total Phenolics
  • Radial Diffusion Assay for Tannins
  • Acid Butanol Assay for Proanthocyanidins (Condensed Tannins)
  • Proximate Lignin and Cellulose
  • Leaf Toughness
  • Microbial Decomposers
  • Techniques for Handling Ingoldian Fungi
  • Maintenance of Aquatic Hyphomycete Cultures
  • An Illustrated Key to the Common Temperate Species of Aquatic Hyphomycetes
  • Molecular Approaches to Estimate Fungal Diversity. I. Terminal Restriction Fragment Length Polymorphism (T-RFLP)
  • Proteinase Activity: Azocoll and Thin-Layer Enzyme Assay
  • Detritivorous Consumers
  • Maintenance of Shredders in the Laboratory
  • Feeding Preferences
  • Data Analysis
  • Biodiversity
  • A Primer for Statistical Analysis
  • Molecular Approaches to Estimate Fungal Diversity. II. Denaturing Gradient Gel Electrophoresis (DGGE)
  • Sporulation by Aquatic Hyphomycetes
  • Ergosterol as a Measure of Fungal Biomass
  • Acetate Incorporation into Ergosterol to Determine Fungal Growth Rates and Production
  • Bacterial Counts and Biomass Determination by Epifluorescence Microscopy
  • Secondary Production and Growth of Litter-Associated Bacteria
  • Isolation of Cellulose-Degrading Bacteria
  • Extraction and Quantification of ATP as a Measure of Microbial Biomass
  • Respirometry
  • Enzymatic Capabilities
  • Extractellular Fungal Hydrolytic Enzyme Activity
  • Cellulases
  • Viscosimetric Determination of Endocellulase Activity
  • Fluorometric Determination of the Activity of ?-Glucosidase and Other Extracellular Hydrolytic Enzymes
  • Pectin-Degrading Enzymes: Polygalacturonase and Pectin Lyase.-Lignin-Degrading Enzymes: Phenoloxidase and Peroxidase
  • Phenol Oxidation