Capillary Electrophoresis of Carbohydrates

Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques i...

Full description

Bibliographic Details
Other Authors: Thibault, Pierre (Editor), Honda, Susumu (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 2003, 2003
Edition:1st ed. 2003
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Structural and Functional Diversity of Glycoconjugates
  • Sample Preparation Methods
  • Chemical and Enzymatic Release of Glycans from Glycoproteins
  • Derivatization of Carbohydrates
  • Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes
  • Analysis of Mono- and Oligosaccharides
  • Monosaccharide Analysis by Capillary Electrophoresis
  • Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
  • Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis
  • Affinity Capillary Electrophoresis of Oligosaccharides
  • Characterization of Glycosaminoglycans by Capillary Electrophoresis
  • Analysis of Glycoconjugates and the Component Monosaccharides
  • Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection
  • Separation of Protein Glycoforms by Capillary Electrophoresis
  • Separation of Glycoproteins by Capillary Isoelectric Focusing
  • Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry
  • Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry
  • Specific Applications
  • Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity
  • Determination of Association Constant of Carbohydrate-Protein Interaction