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090727 ||| eng |
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|a 9781592592081
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|a QH573-671
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|a Moss, Tom
|e [editor]
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|a DNA'Protein Interactions
|h Elektronische Ressource
|b Principles and Protocols
|c edited by Tom Moss
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| 250 |
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|a 2nd ed. 2001
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 2001, 2001
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| 300 |
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|a 656 p. 146 illus
|b online resource
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|a Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast -- Assays for Transcription Factor Activity -- Assay of Restriction Endonucleases Using Oligonucleotides -- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Calorimetry of Protein-DNA Complexes and Their Components -- Surface Plasmon Resonance Applied to DNA-Protein Complexes -- Reconstitution of Protein-DNA Complexes for Crystallization -- Two-Dimensional Crystallization of Soluble Protein Complexes -- Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Scanning Transmission Electron Microscopy of DNA-Protein Complexes -- Determination of Nucleic Acid Recognition Sequences by SELEX -- High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation
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|a Filter-Binding Assays -- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions -- DNase I Footprinting -- Footprinting with Exonuclease III -- Hydroxyl Radical Footprinting -- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA -- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper -- Uranyl Photofootprinting -- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions -- Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots -- Diffusible Singlet Oxygen as a Probe of DNA Deformation -- Ultraviolet-Laser Footprinting -- In Vivo DNA Analysis -- Identification of Protein-DNA Contacts with Dimethyl Sulfate -- Ethylation Interference -- Hydroxyl Radical Interference --
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|a Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates -- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes -- Chemical Modification of Lysine by Reductive Methylation -- Limited Proteolysis of Protein-Nucleic Acid Complexes -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Site-Specific Protein-DNA Photocrosslinking -- Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes -- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex -- UV Laser-Induced Protein-DNA Crosslinking -- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Engineering Nucleic Acid-Binding Proteins by Phage Display --
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| 653 |
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|a Cell Biology
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| 653 |
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|a Cytology
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| 041 |
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|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
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|a 10.1385/1592592082
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| 856 |
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|u https://doi.org/10.1385/1592592082?nosfx=y
|x Verlag
|3 Volltext
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|a 571.6
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| 520 |
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|a DNA-protein interactions are fundamental to understanding biological growth, development, differentiation, evolution, and disease, and are also increasingly important to the biotechnology industry. In DNA-Protein Interactions: Principles and Protocols, 2nd Edition, Dr. Tom Moss assembles the new standard collection of cutting-edge techniques to identify key protein-DNA interactions, define their components, and determine the nature of their interactions and their manner of function, both in the cell and in the test tube. Each detailed method has been optimized and tested by a hands-on expert to ensure experimental success, and provides helpful notes on pitfalls to avoid, as well as alternate strategies. The techniques span a wide range, from factor identification to atomic detail, and include multiple DNA footprinting analyses, including in vivo strategies, gel shift (EMSA) optimization, SELEX, surface plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. There are also vanguard methods for designing proteins to fit DNA, site-specific protein-directed DNA cleavage, and 2D semicrystalline arrays of nucleoproteins for structure determination. Comprehensive and broad ranging, DNA-Protein Interactions: Principles and Protocols, 2nd Edition, offers a stellar array of over 100 up-to-date and readily reproducible techniques that biochemists and molecular, cellular, and developmental biologists can use successfully today to illuminate DNA-protein interactions
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