Integrin Protocols
In Integrin Protocols, Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the R...
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Format: | eBook |
Language: | English |
Published: |
Totowa, NJ
Humana Press
1999, 1999
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Edition: | 1st ed. 1999 |
Series: | Methods in Molecular Biology
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Subjects: | |
Online Access: | |
Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Working with Integrins
- Integrin-Binding Peptides Derived from Phage Display Libraries
- Production of Rat Monoclonal Antibodies Specific for Mouse Integrins
- Biochemical Analysis of Integrin-Mediated Shc Signaling
- Assays for Kinase Activity
- Immunoblotting of Integrins
- Application of Flow Cytometry in the Analysis and Sterile Sorting of Cell Populations Based on Integrin Expression
- Expression of Integrin Transcripts in Human Cancer Cells
- Functional Analysis of Integrins
- Functional Analysis of the ?2 Integrins
- Expression of Heterologous Integrin Genes
- Preparing a Polyclonal Antibody to Mouse ?1 Integrin with Function-Blocking Activity
- Integrin Gene Targeting
- Determination of Positive and Negative Regulatory Elements
- Integrin Function at the Cellular Level
- A Cell-Based Adhesion Assay for the Characterization of Integrin ?v?3 Antagonists
- Integrins in Cell Migration
- Models for Studying Cellular Invasion of Basement Membranes
- Methods for Studying Anoikis
- Use of the 10-Day-Old Chick Embryo Model for Studying Angiogenesis
- Cell Shape and Integrin Signaling Regulate the Differentiation State of Mammary Epithelial Cells