Integrin Protocols

In Integrin Protocols, Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the R...

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Bibliographic Details
Other Authors: Howlett, Anthony R. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1999, 1999
Edition:1st ed. 1999
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Working with Integrins
  • Integrin-Binding Peptides Derived from Phage Display Libraries
  • Production of Rat Monoclonal Antibodies Specific for Mouse Integrins
  • Biochemical Analysis of Integrin-Mediated Shc Signaling
  • Assays for Kinase Activity
  • Immunoblotting of Integrins
  • Application of Flow Cytometry in the Analysis and Sterile Sorting of Cell Populations Based on Integrin Expression
  • Expression of Integrin Transcripts in Human Cancer Cells
  • Functional Analysis of Integrins
  • Functional Analysis of the ?2 Integrins
  • Expression of Heterologous Integrin Genes
  • Preparing a Polyclonal Antibody to Mouse ?1 Integrin with Function-Blocking Activity
  • Integrin Gene Targeting
  • Determination of Positive and Negative Regulatory Elements
  • Integrin Function at the Cellular Level
  • A Cell-Based Adhesion Assay for the Characterization of Integrin ?v?3 Antagonists
  • Integrins in Cell Migration
  • Models for Studying Cellular Invasion of Basement Membranes
  • Methods for Studying Anoikis
  • Use of the 10-Day-Old Chick Embryo Model for Studying Angiogenesis
  • Cell Shape and Integrin Signaling Regulate the Differentiation State of Mammary Epithelial Cells