Free Radical and Antioxidant Protocols

In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible...

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Bibliographic Details
Other Authors: Armstrong, Donald (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1998, 1998
Edition:1st ed. 1998
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Synthesis of Lipid and Cholesterol Hydroperoxide Standards
  • Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography
  • Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo
  • Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC
  • Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA
  • Nitric Oxide Synthase
  • Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR
  • A Fluorescent-Based Assay for Measurement of Phospholipase A2 Activity
  • Autofluorescent Ceroid/Lipofuscin
  • In Vivo Technique for Autofluorescent Lipopigments
  • An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism
  • Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion
  • Techniques for the Measurement of Antioxidant Activity
  • Simultaneous Determination of Serum Retinol, Tocopherols, and Carotenoids by HPLC
  • EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E
  • Nonvitamin Plasma Antioxidants
  • Regulatory Antioxidant Enzymes
  • In Vitro Screening for Antioxidant Activity
  • Antioxidant Activity of Low-Density Lipoprotein
  • Lipoic Acid as an Antioxidant
  • Reduced Glutathione and Glutathione Disulfide
  • Analysis of Coenzyme Q10 Content in Human Plasma and Other Biological Samples
  • Techniques Applicable to Both Oxidative Stress and Antioxidant Activity
  • A Simple Luminescence Method for Detecting Lipid Peroxidation and Antioxidant Activity In Vitro
  • Analysis of Oxidation and Antioxidants Using Microtiter Plates
  • Trace Element Analysis of Biological Samples by Analytical Atomic Spectroscopy
  • Energy Dispersive X-Ray Microanalysis
  • Pro-Oxidant and Antioxidant Effects of Estrogens
  • Artificial Radical Generating and Scavenging Systems
  • Techniques for the Measurement of Oxidative Stress
  • Oxygen Consumption Methods
  • Spin Trapping and Electron Paramagnetic Resonance Spectroscopy
  • In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy
  • Single Photon Counting
  • Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence
  • Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography
  • Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid
  • Aromatic Hydroxylation
  • Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma
  • Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma
  • Antibodies Against Malondialdehyde-Modified Proteins
  • Oxidized LDL and Lp(a)
  • Oxidized and Unoxidized Fatty Acyl Esters