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090727 ||| eng |
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|a 9781592592548
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|a QD415-436
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| 100 |
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|a Armstrong, Donald
|e [editor]
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| 245 |
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|a Free Radical and Antioxidant Protocols
|h Elektronische Ressource
|c edited by Donald Armstrong
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| 250 |
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|a 1st ed. 1998
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| 260 |
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|a Totowa, NJ
|b Humana Press
|c 1998, 1998
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| 300 |
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|a XVII, 455 p
|b online resource
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|a Synthesis of Lipid and Cholesterol Hydroperoxide Standards -- Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography -- Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo -- Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC -- Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA -- Nitric Oxide Synthase -- Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR -- A Fluorescent-Based Assay for Measurement of Phospholipase A2 Activity -- Autofluorescent Ceroid/Lipofuscin -- In Vivo Technique for Autofluorescent Lipopigments -- An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism -- Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion -- Techniques for the Measurement of Antioxidant Activity --
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|a Simultaneous Determination of Serum Retinol, Tocopherols, and Carotenoids by HPLC -- EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E -- Nonvitamin Plasma Antioxidants -- Regulatory Antioxidant Enzymes -- In Vitro Screening for Antioxidant Activity -- Antioxidant Activity of Low-Density Lipoprotein -- Lipoic Acid as an Antioxidant -- Reduced Glutathione and Glutathione Disulfide -- Analysis of Coenzyme Q10 Content in Human Plasma and Other Biological Samples -- Techniques Applicable to Both Oxidative Stress and Antioxidant Activity -- A Simple Luminescence Method for Detecting Lipid Peroxidation and Antioxidant Activity In Vitro -- Analysis of Oxidation and Antioxidants Using Microtiter Plates -- Trace Element Analysis of Biological Samples by Analytical Atomic Spectroscopy -- Energy Dispersive X-Ray Microanalysis -- Pro-Oxidant and Antioxidant Effects of Estrogens -- Artificial Radical Generating and Scavenging Systems
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| 505 |
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|a Techniques for the Measurement of Oxidative Stress -- Oxygen Consumption Methods -- Spin Trapping and Electron Paramagnetic Resonance Spectroscopy -- In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy -- Single Photon Counting -- Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence -- Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography -- Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid -- Aromatic Hydroxylation -- Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma -- Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma -- Antibodies Against Malondialdehyde-Modified Proteins -- Oxidized LDL and Lp(a) -- Oxidized and Unoxidized Fatty Acyl Esters --
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| 653 |
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|a Biochemistry
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| 041 |
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|a eng
|2 ISO 639-2
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| 989 |
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|b SPRPROT
|a Springer Protocols Archive 1981-2004
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| 490 |
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|a Methods in Molecular Biology
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| 028 |
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|a 10.1385/0896034720
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| 856 |
4 |
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|u https://doi.org/10.1385/0896034720?nosfx=y
|x Verlag
|3 Volltext
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| 082 |
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|a 572
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| 520 |
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|a In Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process
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