Mycobacteria Protocols
In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques,...
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Format: | eBook |
Language: | English |
Published: |
Totowa, NJ
Humana Press
1998, 1998
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Edition: | 1st ed. 1998 |
Series: | Methods in Molecular Biology
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Subjects: | |
Online Access: | |
Collection: | Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa |
Table of Contents:
- Mycobacteria
- Mycobacteria
- Isolation of Genomic DNA from Mycobacteria
- Nucleic-Acid Extraction: Plasmid/Cosmid DNA
- Pulsed Field Gel Electrophoresis for Mycobacteria
- Extraction of RNA from Mycobacteria
- Preparation of Cell-Free Extracts from Mycobacteria
- Preparation of Cell-Wall Fractions from Mycobacteria
- Using MycDB on the World-Wide Web
- Conjugating DNA into Mycobacteria
- Electroporation of Mycobacteria
- Mycobacteriophages
- Chemical Mutagenesis of Mycobacteria
- Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
- Homologous Recombination in Mycobacterium smegmatis
- Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker
- Gene Replacement in Mycobacterium intracellulare
- Gene Replacement in Mycobacterium bovis BCG
- The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria
- Green Fluorescent Protein
- Expression of Genes in Mycobacteria
- Secretion of Mammalian Proteins from Mycobacteria
- Intracellular Gene Expression
- 24 Analysis of Mycobacterial Differential Gene Expression by RAP-PCR
- Mycobacterial Speciation
- Species Identification of Mycobacteria Using rDNA Sequencing
- Detection of Mycobacterium tuberculosis by a Calorimetric Polymerase Chain Reaction
- Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping
- Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis
- Detection of Mutations in Mycobacteria by PCR-SSCP (Single-Strand Conformation Polymorphism)
- The Use of Luciferase-Reporter Phage for Antibiotic-Susceptibility Testing of Mycobacteria