Mycobacteria Protocols

In Mycobacteria Protocols, laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease-including tuberculosis and leprosy. The methods range from basic culture techniques,...

Full description

Bibliographic Details
Other Authors: Parish, Tanya (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1998, 1998
Edition:1st ed. 1998
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Mycobacteria
  • Mycobacteria
  • Isolation of Genomic DNA from Mycobacteria
  • Nucleic-Acid Extraction: Plasmid/Cosmid DNA
  • Pulsed Field Gel Electrophoresis for Mycobacteria
  • Extraction of RNA from Mycobacteria
  • Preparation of Cell-Free Extracts from Mycobacteria
  • Preparation of Cell-Wall Fractions from Mycobacteria
  • Using MycDB on the World-Wide Web
  • Conjugating DNA into Mycobacteria
  • Electroporation of Mycobacteria
  • Mycobacteriophages
  • Chemical Mutagenesis of Mycobacteria
  • Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System
  • Homologous Recombination in Mycobacterium smegmatis
  • Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker
  • Gene Replacement in Mycobacterium intracellulare
  • Gene Replacement in Mycobacterium bovis BCG
  • The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria
  • Green Fluorescent Protein
  • Expression of Genes in Mycobacteria
  • Secretion of Mammalian Proteins from Mycobacteria
  • Intracellular Gene Expression
  • 24 Analysis of Mycobacterial Differential Gene Expression by RAP-PCR
  • Mycobacterial Speciation
  • Species Identification of Mycobacteria Using rDNA Sequencing
  • Detection of Mycobacterium tuberculosis by a Calorimetric Polymerase Chain Reaction
  • Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping
  • Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis
  • Detection of Mutations in Mycobacteria by PCR-SSCP (Single-Strand Conformation Polymorphism)
  • The Use of Luciferase-Reporter Phage for Antibiotic-Susceptibility Testing of Mycobacteria