Electroporation Protocols for Microorganisms

Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and...

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Bibliographic Details
Other Authors: Nickoloff, Jac A. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1995, 1995
Edition:1st ed. 1995
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Electroporation Theory
  • Instrumentation
  • Direct Plasmid Transfer Between Bacterial Species and Electrocuring
  • Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation
  • Production of cDNA Libraries by Electroporation
  • Electroporation of RNA into Saccharomyces cerevisiae
  • Electrofusion of Yeast Protoplasts
  • Escherichia coli Electrotransformation
  • Electrotransformation in Salmonella
  • Electrotransformation of Pseudomonas
  • Electroporation of Xanthomonas
  • Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids
  • Electroporation of Francisella tularensis
  • A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation
  • Genetic Transformation of Bacteroides spp. Using Electroporation
  • Electrotransformation of Agrobacterium
  • Electroporation of Helicobacter pylori
  • Electrotransformation of Streptococci
  • Transformation of Lactococcus by Electroporation
  • Transformation of Lactobacillus by Electroporation
  • Electrotransformation of Staphylococci
  • Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine
  • of Recombinant DNA into Clostridium spp.
  • Electroporation of Mycobacteria
  • Electrotransformation of the Spirochete Borrelia burgdorferi
  • Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation
  • Ten-Minute Electrotransformation of Saccharomyces cerevisiae
  • Electroporation of Schizosaccharomyces pombe
  • Gene Transfer by Electroporation of Filamentous Fungi
  • Transformation of Candida maltosa by Electroporation
  • Electroporation of Physarum polycephalum
  • Electroporation of Dictyostelium discoideum
  • Gene Transfer by Electroporation in Tetrahymena
  • Transfection of the African and American Trypanosomes
  • Electroporation in Giardia lamblia