Diagnostic Bacteriology Protocols

Enhanced public awareness of microbiological issues and increased governmental regulation have ensured that microbiology and bacteriology remain important, from the academic laboratory to a broad range of indus­ trial environments. Since the techniques used in bacteriology vary widely and have advan...

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Bibliographic Details
Main Authors: Howard, Jenny, Whitcombe, David M. (Author)
Format: eBook
Language:English
Published: Totowa, NJ Humana Press 1995, 1995
Edition:1st ed. 1995
Series:Methods in Molecular Biology
Subjects:
Online Access:
Collection: Springer Protocols Archive 1981-2004 - Collection details see MPG.ReNa
Table of Contents:
  • Antibiotic Resistance Profiling
  • Bacteriocin Typing
  • Bacteriophage Typing
  • The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis
  • Lipopolysaccharide Chemotyping
  • Analysis of Bacterial Outer Membrane Proteins
  • Multilocus Enzyme Electrophoresis
  • Fast Atom Bombardment-Mass Spectrometry
  • Pyrolysis Mass Spectrometry
  • The Direct Immunofluorescent Filter Technique (DIFT)
  • The Direct Epifluorescent Filter Technique (DEFT)
  • Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens
  • Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format
  • Preparation of Bacterial Genomic DNA
  • Pulsed Field Gel Electrophoresis
  • Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains
  • Determination of rRNA Gene Restriction Patterns
  • Preparation of Nonradioactive DNA Probes
  • Preparation of Bacterial Plasmid DNA
  • Plasmid Profile Typing and Plasmid Fingerprinting
  • Polymerase Chain Reaction or the Detection of Listeria Species and Listeria monocytogenes
  • Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR
  • Identification of Microorganisms Using Random Primed PCR
  • Ligase Chain Reaction